In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants

Abstract

A protocol for in vitro multiplication of Capparis decidua (Forsk.) Edgew. has been developed from cultured leaves procured from multiplying axillary shoots on the cultured nodal explants. The highest efficiency of shoot formation was observed on Murashige and Skoog (MS) medium containing 2 mg dm-3 benzyladenine (BA) and 0.5 mg dm-3 1-naphthaleneacetic acid. The regenerated shoots were transferred to MS medium containing 3 mg dm-3 BA for growth and proliferation. Shoots above 2 cm in length were transferred to MS medium supplemented with 1 mg dm-3 indole-3-butyric acid plus 0.5 mg dm-3 indole-3-acetic acid for root induction. No variation was detected among the micropropagated plants by randomly amplified polymorphic DNA (RAPD) markers. © Springer Science+Business Media B.V 2010.