Effect of varying dietary protein levels on hematology of Labeo calbasu

Abstract

The present work is the first detailed report from Pakistan on study of blood parameters in Labeo calbasu, under three treatments (T1, T2 and T3) fed at different crude protein (CP) levels ratios i.e., 15%, 20% and 25% CP. Fish were first collected, acclimatized, kept in triplicate aquaria under controlled conditions in three groups (T1, T2 and T3) and then 5 fish were selected from each treatment at the end of 12 weeks of feeding trial from January to April, 2018, during which fish were fed at the rate of 4% of their body weight. The blood variants were studied like Lymphocytes (LYM), Monocytes (MON), Granulocytes (GRA), Red Blood Cells (R.B.C), Hemoglobin (Hgb), Hematocrit (HCT), Mean Corpuscular volume (MCV), Mean Corpuscular Haemoglobin (MCH), Red Blood Cell Distribution Width when measured with Standard Deviation (RDW-SD), Mean Corpuscular Haemoglobin Concentration (MCHC), Platelets (PLT), Plateletcrit (PCT), Mean Platelets Volume (MPV), Platelets Distribution Width (PDW) For statistical analysis, regression was applied to the data. Fish fed with 25% dietary protein level under T3 showed good health status as compared to T1 and T2 with 15% and 20% dietary protein levels. Results of the research work indicated that L. calbasu reared by feeding 25%CP showed better health status.