Biological activity of Bt proteins expressed in different structures of transgenic corn against Spodoptera frugiperda

  • Bernardi D
  • Bernardi O
  • Horikoshi R
  • et al.
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Abstract

ABSTRACT: Spodoptera frugiperda (J. E. Smith) is the main target pest of Bt corn technologies, such as YieldGard VT PRO(tm) (Cry1A.105/Cry2Ab2) and PowerCore(tm) (Cry1A.105/Cry2Ab2/Cry1F). In this study, it was evaluated the biological activity of Bt proteins expressed in different plant structures of YieldGard VT PRO(tm) and PowerCore(tm) corn against S. frugiperda . Complete mortality of S. frugiperda neonates was observed on leaf-disc of both Bt corn technologies. However, the mortality in silks and grains was lower than 50 and 6%, respectively. In addition, more than 49% of the surviving larvae in silks and grains completed the biological cycle. However, all life table parameters were negatively affected in insects that developed in silks and grains of both Bt corn events. In summary, the low biological activity of Bt proteins expressed on silks and grains of YieldGard VT PRO(tm) and PowerCore(tm) corn can contribute to the resistance evolution in S. frugiperda populations.RESUMO: Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae ) é a principal praga-alvo dos eventos de milho YieldGard VT PRO(tm) (Cry1A.105/Cry2Ab2) e PowerCore(tm) (Cry1A.105/Cry2Ab2/Cry1F). Para subsidiar o manejo de resistência, avaliou-se a atividade biológica das proteínas de Bt expressas em diferentes estruturas dessas tecnologias de milho Bt contra S. frugiperda . Em discos de folha de milho YieldGard VT PRO(tm) e PowerCore(tm), houve mortalidade completa de neonatas de S. frugiperda . No entanto, em estilo-estigmas e grãos, a mortalidade foi inferior a 50% e 6%, respectivamente. Em adição, mais de 49% das larvas sobreviventes em estilos-estigmas e grãos completaram o ciclo biológico. No entanto, todos os parâmetros de tabela de vida de fertilidade foram negativamente afetados nos insetos que se desenvolveram em estilos-estigmas e grãos de milho Bt. A baixa atividade biológica das proteínas Bt expressas em estilos-estigmas e grãos de milho YieldGard VT PRO(tm) e PowerCore(tm) pode contribuir para a evolução de resistência em populações de S. frugiperda .

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APA

Bernardi, D., Bernardi, O., Horikoshi, R. J., Salmeron, E., Okuma, D. M., & Omoto, C. (2016). Biological activity of Bt proteins expressed in different structures of transgenic corn against Spodoptera frugiperda. Ciência Rural, 46(6), 1019–1024. https://doi.org/10.1590/0103-8478cr20150047

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