Despite some important limitations, immunocompetent mouse models of HBV replication remain an essential tool for studying cellular and humoral immunity to the virus. CD8+ T cells are a critical component of the immune response to HBV due to their ability to both kill virus-infected hepatocytes and produce cytokines such as IFN-γ that non-cytopathically inhibit virus replication. A number of techniques can be used to measure the magnitude, specificity, and functionality of HBV-specific CD8+ T cells, each having its own unique advantages. We describe here the enzyme-linked immunospot (ELISPOT)-based assay, which, compared to other methods, is sensitive, cost-effective, and rapid and requires relatively little optimization, specialized training, or equipment.
CITATION STYLE
Reynolds, T. D., Moshkani, S., & Robek, M. D. (2017). An ELISPOT-based assay to measure HBV-specific CD8+ T cell responses in immunocompetent mice. In Methods in Molecular Biology (Vol. 1540, pp. 237–247). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6700-1_20
Mendeley helps you to discover research relevant for your work.