Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR

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Abstract

Long range distance measurement on RNA allow the determination of RNA folds. Here we report the site specific incorporation of nitroxide spin labels at U,C and A by "on column synthesis". PELDOR (Pulsed Electron Double Resonance) measurements of several RNAs in the range of 2-6 nm were successful.

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Frolow, O., Endeward, B., Schiemann, O., Prisner, T. F., & Engels, J. W. (2008). Nitroxide spin labeled RNA for long range distance measurements by EPR-PELDOR. Nucleic Acids Symposium Series (2004), (52), 153–154. https://doi.org/10.1093/nass/nrn078

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