Self-healing biogeopolymers using biochar-immobilized spores of pure-and co-cultures of bacteria

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Abstract

A sustainable solution for crack maintenance in geopolymers is necessary if they are to be the future of modern green construction. This study aims to develop self-healing biogeopolymers that could potentially rival bioconcrete. First, a suitable healing agent was selected from Bacillus subtilis, Bacillus sphaericus, and Bacillus megaterium by directly adding their spores in the geopolymers and subsequently exposing them to a precipitation medium for 14 days. Scanning electron microscope with energy-dispersive X-ray (SEM-EDX) analysis revealed the formation of mineral phases for B. subtilis and B. sphaericus. Next, the effect of biochar-immobilization and co-culturing (B. sphaericus and B. thuringiensis) on the healing efficiencies of the geopolymers were tested and optimized by measuring their ultrasonic pulse velocities weekly over a 28-day healing period. The results show that using co-cultured bacteria significantly improved the observed efficiencies, while biochar-immobilization had a weak effect, but yielded an optimum response between 0.3–0.4 g/mL. The maximum crack width sealed was 0.65 mm. Through SEM-EDX and FTIR analyses, the precipitates in the cracks were identified to be mainly CaCO3. With that, there is potential in developing self-healing biogeopolymers using biochar-immobilized spores of bacterial cultures.

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CITATION STYLE

APA

Doctolero, J. Z. S., Beltran, A. B., Uba, M. O., Tigue, A. A. S., & Promentilla, M. A. B. (2020). Self-healing biogeopolymers using biochar-immobilized spores of pure-and co-cultures of bacteria. Minerals, 10(12), 1–17. https://doi.org/10.3390/min10121114

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