Rapid separation of bovine whey proteins by membrane convective liquid chromatography, perfusion chromatography, continuous bed chromatography, and capillary electrophoresis

Abstract

Membrane convective liquid chromatography is a technique based on porous cellulose membranes designed for the separation of biomolecules in few minutes at high flow-rates and low back-pressures. Bovine whey proteins are separated in less than 10 min, at pH 8.5, with a flow-rate of 5.6 mL·min-1 and with a 0-0.2 mol·L-1 NaCl linear gradient. Three other rapid methods are also proposed. With the ion-exchange perfusion liquid chromatography based on beads with large pores and with the continuous bed chromatography based on a polymer matrix, separations are achieved in only 10 min. Capillary zone electrophoresis using an untreated fused-silica capillary allows the separation of whey proteins in a single run of 8 min without the presence of polymeric additives. These rapid methods are suitable in the quality control of wheys and could be applied in dairy industry or in research. © Inra/Elsevier, Paris.