Chemical Structure of the 2-Keto-3-Deoxyoctonate (KDO) Region of the Lipopolysaccharide Isolated from Ol Vibrio cholerae NIH 4IR (Ogawa)

Abstract

The chemical structure of the 2-keto-3-deoxyoctonate (KDO) region of the lipopolysaccharide (LPS) isolated from 01 V. cholerae NIH 41R (Ogawa) was elucidated by dephosphorylation, periodate oxidation and methylation analysis. Methylation analysis of KDO in the dephosphorylated LPS revealed the presence of 5-0-acetyl-l,2,4,6,7,8-hexa-0-methyl-3~deoxy~octitol and 2-keto-3-deoxy-heptulo-sonic acid was detected in the methanolysate of the periodate-oxidized and dephos-phorylated LPS. These results indicated that the site of binding of KDO to the core oligosaccharide is position C5 as in enteric gram-negative bacterial LPS, while only one molecule of the KDO residue carrying phosphate on position C4 is present in the inner core region of the LPS in contrast to enteric gram-negative bacterial LPS in which one molecule of KDO carrying KDO or KD02—>-4KDO disaccharide instead of the phosphate group at position C4 is present in its main chain. © 1991, Center For Academic Publications Japan. All rights reserved.