Evaluation of different extraction methods of phytochemical and antioxidant compounds of Moringa oleifera leaf extract

Abstract

Moringa oleifera is known as a rich and cost-effective source of phytochemical compounds and has many applications in industry, water treatment, pharmacy and biofuels The leaves of Moringa oleifera were introduced by the Taiwan Plant Center as one of the 120 food species studied as the plant with the highest nutritional content. In this study, the effect of different extraction methods such as Maceration and Soxhlet on phenolic content, flavonoid content and antioxidant properties, The effect of these extracts on A549 lung cancer cells was also evaluated by MTT assay. This plant was studied. According to the results obtained from the leaves of Moringa oleifera, the highest extraction efficiency was related to Soxhlet extraction. The highest phenolic and flavonoid content was related to Maceration extraction method. The results of studying the antioxidant properties of the extract showed that most of the antioxidant properties were related to the extract obtained by Soxhlet method. MTT test showed that among the various extracts, the lowest IC50 was related to the extract extracted by Soxhlet method. This study shows that Moringa oleifera leaves are a good nutritional source.