In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant

45Citations
Citations of this article
40Readers
Mendeley users who have this article in their library.

Abstract

This is the first attempt towards an efficient regeneration protocol for an endangered and valuable medicinal plant, Thymus persicus using in vitro callus induction and indirect organogenesis. Callus induction was performed on MS medium supplemented with different concentrations of NAA and 2,4-D, alone or in combination with BAP and KN. Maximum callus induction (100%) was achieved from internode explants cultured on MS medium fortified with 2.0 mg L–1 NAA and 0.5 mg L–1 KN. The highest frequency of shoot multiplication (96%) was observed with 2.0 mg L–1 BAP+1.0 mg L–1 NAA. The maximum number of rootlets (16.6 ± 1.4) was induced on half-strength MS medium with 0.5 and 1.0 mg L–1 IBA. Rooted plantlets were then successfully grown and acclimatized in the greenhouse with a 70-85% survival rate. The benefits of the protocol described here include all-year-round application, germplasm conservation, suitability for commercial production and also for the biotechnological production of pentacyclic triterpenoids.

Cite

CITATION STYLE

APA

Bakhtiar, Z., Mirjalili, M. H., & Sonboli, A. (2016). In vitro callus induction and micropropagation of Thymus persicus (Lamiaceae), an endangered medicinal plant. Crop Breeding and Applied Biotechnology, 16(1), 48–54. https://doi.org/10.1590/1984-70332016v16n1a8

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free