Tissue factor upregulation is associated with SARS-CoV-2 in the lungs of COVID-19 patients

35Citations
Citations of this article
37Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Background: A substantial proportion of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) develop severe/critical coronavirus disease 2019 (COVID-19) characterized by acute respiratory distress syndrome (ARDS) with thrombosis. Objectives: We tested the hypothesis that SARS-CoV-2--induced upregulation of tissue factor (TF) expression may be responsible for thrombus formation in COVID-19. Methods: We compared autopsy lung tissues from 11 patients with COVID-19--associated ARDS with samples from 6 patients with ARDS from other causes (non-COVID-19 ARDS) and 11 normal control lungs. Results: Dual RNA in situ hybridization for SARS-CoV-2 and TF identified sporadic clustered SARS-CoV-2 with prominent co-localization of SARS-CoV-2 and TF RNA. TF expression was 2-fold higher in COVID-19 than in non-COVID-19 ARDS lungs (P =.017) and correlated with the intensity of SARS-CoV-2 staining (R2 =.36, P =.04). By immunofluorescence, TF protein expression was 2.1-fold higher in COVID-19 versus non-COVID-19 ARDS lungs (P =.0048) and 11-fold (P

Cite

CITATION STYLE

APA

Subrahmanian, S., Borczuk, A., Salvatore, S., Fung, K. M., Merrill, J. T., Laurence, J., & Ahamed, J. (2021). Tissue factor upregulation is associated with SARS-CoV-2 in the lungs of COVID-19 patients. Journal of Thrombosis and Haemostasis, 19(9), 2268–2274. https://doi.org/10.1111/jth.15451

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free