CpG islands are regions of vertebrate genomes that often function as gene regulatory elements and are associated with most gene promoters. CpG island elements usually contain nonmethylated CpG dinucleotides, while the remainder of the genome is pervasively methylated. We developed a biochemical approach called biotinylated CxxC affinity purification (BioCAP) to unbiasedly isolate regions of the genome that contain nonmethylated CpG dinucleotides. The resulting highly pure nonmethylated DNA is easily analyzed by quantitative PCR to interrogate specific loci or via massively parallel sequencing to yield genome-wide profiles.
CITATION STYLE
Long, H. K., Rose, N. R., Blackledge, N. P., & Klose, R. J. (2018). Biochemical identification of nonmethylated DNA by BioCAP-seq. In Methods in Molecular Biology (Vol. 1766, pp. 15–29). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7768-0_2
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