Human B-1 and B-2 B Cells Develop from Lin−CD34+CD38lo Stem Cells

  • Quách T
  • Hopkins T
  • Holodick N
  • et al.
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Abstract

The B-1 B cell population is an important bridge between innate and adaptive immunity primarily because B-1 cells produce natural Ab. Murine B-1 and B-2 cells arise from distinct progenitors; however, in humans, in part because it has been difficult to discriminate between them phenotypically, efforts to pinpoint the developmental origins of human B-1 and B-2 cells have lagged. To characterize progenitors of human B-1 and B-2 cells, we separated cord blood and bone marrow Lin−CD34+ hematopoietic stem cells into Lin−CD34+CD38lo and Lin−CD34+CD38hi populations. We found that transplanted Lin−CD34+CD38lo cells, but not Lin−CD34+CD38hi cells, generated a CD19+ B cell population after transfer into immunodeficient NOD.Cg-Prkdcscid Il2rgtm1wjl/SxJ neonates. The emergent CD19+ B cell population was found in spleen, bone marrow, and peritoneal cavity of humanized mice and included distinct populations displaying the B-1 or the B-2 cell phenotype. Engrafted splenic B-1 cells exhibited a mature phenotype, as evidenced by low-to-intermediate expression levels of CD24 and CD38. The engrafted B-1 cell population expressed a VH-DH-JH composition similar to cord blood B-1 cells, including frequent use of VH4-34 (8 versus 10%, respectively). Among patients with hematologic malignancies who underwent hematopoietic stem cell transplantation, B-1 cells were found in the circulation as early as 8 wk posttransplantation. Altogether, our data demonstrate that human B-1 and B-2 cells develop from a Lin−CD34+CD38lo stem cell population, and engrafted B-1 cells in humanized mice exhibit an Ig-usage pattern comparable to B-1 cells in cord blood.

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APA

Quách, T. D., Hopkins, T. J., Holodick, N. E., Vuyyuru, R., Manser, T., Bayer, R.-L., & Rothstein, T. L. (2016). Human B-1 and B-2 B Cells Develop from Lin−CD34+CD38lo Stem Cells. The Journal of Immunology, 197(10), 3950–3958. https://doi.org/10.4049/jimmunol.1600630

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