B-cells promote intra-islet CD8+ cytotoxic T-cell survival to enhance type 1 diabetes

Abstract

OBJECTIVE-To determine the role of B-cells in promoting CD8+ T-cell-mediated β cell destruction in chronically inflamed islets. RESEARCH DESIGN AND METHODS-RIP-TNFα-NOD mice were crossed to B-cell-deficient NOD mice, and diabetes development was monitored. We used in vitro antigen presentation assays and in vivo administration of bromodeoxyuridine coupled to flow cytometry assays to assess intra-islet T-cell activation in the absence or presence of B-cells. CD4+Foxp3+ activity in the absence or presence of B-cells was tested using in vivo depletion techniques. Cytokine production and apoptosis assays determined the capacity of CD8+ T-cells transform to cytotoxic T-lymphocytes (CTLs) and survive within inflamed islets in the absence or presence of B-cells. RESULTS-B-cell deficiency significantly delayed diabetes development in chronically inflamed islets. Reintroduction of B-cells incapable of secreting immunoglobulin restored diabetes development. Both CD4+ and CD8+ T-cell activation was unimpaired by B-cell deficiency, and delayed disease was not due to CD4+Foxp3+ T-cell suppression of T-cell responses. Instead, at the CTL transition stage, B-cell deficiency resulted in apoptosis of intra-islet CTLs. CONCLUSIONS-In inflamed islets, B-cells are central for the efficient intra-islet survival of CTLs, thereby promoting type 1 diabetes development. © 2008 by the American Diabetes Association.