Two-step RT-qPCR analysis of expression of 7 drought-related genes in tomato (Lycopersicon esculentum Mill.)

Abstract

The identification and characterization of genes induced under drought stress is a common approach to elucidate the molecular mechanisms of drought stress tolerance in plants. Examination of gene expression using quantitative PCR (qPCR) in combination with Reverse Transcription (RT) in plant responses to drought stress can provide valuable information for stress-tolerance improvement. The purpose of this manuscript is to describe procedure for two step RT-qPCR analysis of gene expression in tomato leaves, under control conditions and under drought stress. Described protocol can be adjusted and used for gene expression analysis of different plant species.