The effects of mushroom components on the proliferation of HeLa cell line in vitro

Abstract

In order to find out nutritional effects of Korean edible mushrooms on the multiplication of tissue cells, the alcohol extracts and acid hydrolysates of eleven species of mushrooms were added to Earle's BSS. A comparison of the respective multiplications of HeLa cells in this solution and in control solution of TC-199. Yielded the following results: The alcohol extracts and acid hydrolysates of Coprinus comatus, Agaricus campestris, Agaricus bisporus, Lentinus edodes, Tricholoma matsutake, Pleurotus ostreatus, Ramaria botrytis and Pholiota nameko influenced favorably the maintenance of the normal form and monolayer of HeLa cells. The growth curves of HeLa cells in the cultures containing, respectively, the alcohol extracts and acid hydrolysates of these eight mushrooms showed that five species, i.e., Coprinus comatus, Agaricus campestris, Agaricus bisporus, Lentinus edodes and Tricholoma matsutake effected an excellent multiplication and that the other three species were less effective than those five species. As to the effects on the cell multiplication, no marked difference was observed between the alcohol extracts and the acid hydrolysates of the mushrooms tested. © 1979 The Pharmaceutical Society of Korea.