Cofactor profi ling of the glucocorticoid receptor from a cellular environment

Abstract

The Microarray Assay for Realtime Coregulator-Nuclear receptor Interaction (MARCoNI) technology allows the identifi cation of nuclear receptor–coregulator interactions via fl ow-through microarrays. As such, differences in the coregulator profi le between distinct nuclear receptors or of a single receptor in agonist or antagonist mode can be investigated, even in a single run. In this chapter, the method how to perform these peptide microarrays with cell lysates containing the overexpressed glucocorticoid receptor is described, as well as the infl uence of assay parameters, variations to the protocol, and data analysis.