The CRISPR/Cas9 system has recently been engineered to confer resistance to geminiviruses in plants. However, we show here that the usefulness of this antiviral strategy is undermined by off-target effects identified by deep sequencing in Arabidopsis. We construct two virus-inducible CRISPR/Cas9 vectors that efficiently inhibit beet severe curly top virus (BSCTV) accumulation in both transient assays (Nicotiana benthamiana) and transgenic lines (Arabidopsis). Deep sequencing detects no off-target effect in candidate sites of the transgenic Arabidopsis. This kind of virus-inducible genome-editing system should be widely applicable for generating virus-resistant plants without off-target costs.
CITATION STYLE
Ji, X., Si, X., Zhang, Y., Zhang, H., Zhang, F., & Gao, C. (2018). Conferring DNA virus resistance with high specificity in plants using virus-inducible genome-editing system. Genome Biology, 19(1). https://doi.org/10.1186/s13059-018-1580-4
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