Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae

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Abstract

Objective: To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously. Methods: Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method. Results: According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI. Conclusions: Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran. © 2013 Hainan Medical College.

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Imani, F. A. A., Iman, I. D., Hosseini, D. R., Karami, A., & Marashi, S. M. (2013). Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae. Asian Pacific Journal of Tropical Medicine, 6(2), 115–118. https://doi.org/10.1016/S1995-7645(13)60005-X

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