Many developmental signals are associated with changes in proliferative response. Also, growing organs and tissues can contain different cellular subpopulations with a defined status in the cell cycle, e.g., quiescent in stem cells, high proliferation in progenitors, cell cycle exit in differentiating cells. This chapter describes a method for isolation of individual cell populations from the Xenopus tadpole brain and determination of their cell cycle status using flow cytometry.
CITATION STYLE
Noelanders, R., & Vleminckx, K. (2018). Cell cycle analysis of the embryonic brain of fluorescent reporter Xenopus tropicalis by flow cytometry. In Methods in Molecular Biology (Vol. 1865, pp. 243–250). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8784-9_17
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