Measuring Circadian Oscillations of Cytosolic-Free Calcium in Arabidopsis thaliana

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Abstract

Circadian oscillations of cytosolic-free calcium can be measured in plants by observing luminescence of the bioluminescent calcium binding protein aequorin. Here we describe the use of intensified photon-counting CCD cameras to measure circadian oscillations in aequorin bioluminescence from Arabidopsis thaliana.

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Hearn, T. J., & Webb, A. A. R. (2014). Measuring Circadian Oscillations of Cytosolic-Free Calcium in Arabidopsis thaliana. Methods in Molecular Biology, 1158, 215–226. https://doi.org/10.1007/978-1-4939-0700-7_15

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