Monoclonal Antibody Analysis of Common Surface Protein(s) of Renibacterium salmoninarum

Abstract

We are reporting the development of two monoclonal antibodies which recognize ten separate isolates of Renibacterium salmoninarum. Both monoclonal antibodies recognize a 57 kDa protein doublet and several lower molecular weight antigens present in bacterial lysates. The 57 kDa antigen appears to correlate with a 57 kDa surface protein named Antigen F by Getchell et al. (1985). Antibody capture ELISA analysis indicates that the antibodies recognize different epitopes of the same protein(s). Cross-reactions were not observed with control bacteria or with normal chinook salmon serum proteins. © 1989, The Japanese Society of Fish Pathology. All rights reserved.