Embryonic stem (ES) cells are unique models for investigating early development and cell differentiation. First identified in mouse and later in other mammals, these cells have also been isolated in avian species. Here, using chicken as a model, we describe a set of protocols allowing the isolation, maintenance, genetic modification, differentiation, and injection of the chicken embryonic stem (cES) cells into embryos for obtaining chimeric animals. © 2013 Springer Science+Business Media, New York.
CITATION STYLE
Aubel, P., & Pain, B. (2013). Chicken embryonic stem cells: Establishment and characterization. Methods in Molecular Biology, 1074, 137–150. https://doi.org/10.1007/978-1-62703-628-3_11
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