Genetically modified (GM) mice are widely used in biomedical research because they can address complex questions in an in-vivo setting that could not otherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.
CITATION STYLE
Delerue, F., & Ittner, L. M. (2022). Microinjection of Zygotes for CRISPR/Cas9-Mediated Insertion of Transgenes into the Murine Rosa26 Safe Harbor. In Methods in Molecular Biology (Vol. 2495, pp. 115–128). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2301-5_7
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