Physical mapping of rDNA genes, (TTAGGG)(n) telomeric sequence and other karyological features in two earthworms of the family Lumbricidae (Annelida: Oligochaeta)

Abstract

A cytogenetical study was carried out on the chromosomes and nuclear DNA amounts of the terrestrial earthworms Octodrilus complanatus and Eisenia foetida (Annelida: Oligochaeta: Lumbricidae). Chromosomes were studied using Giemsa staining, banding methods and fluorescent in situ hybridization (FISH) with two repetitive DNA probes [rDNA and (TTAGGG)(n)]. rDNA FISH and silver staining consistently identified one chromosome pair per spread in both species. The telomeric sequence (TTAGGG)(n) hybridized with termini of all the chromosomes in both earthworms. Flow cytometry DNA assays showed that O. complanatus and E. foetida had different nuclear DNA contents (2C value = 1.72 and = 1.40 pg, respectively) but very similar base composition in their genomes.