RCC1 isoforms differ in their affinity for chromatin, molecular interactions and regulation by phosphorylation

Abstract

RCC1 is the guanine nucleotide exchange factor for Ran GTPase. Generation of Ran-GTP by RCC1 on chromatin provides a spatial signal that directs nucleocytoplasmic transport, mitotic spindle assembly and nuclear envelope formation. We show that RCC1 is expressed in human cells as at least three isoforms, named RCC1α, RCC1β and RCC1γ, which are expressed at different levels in specific tissues. The β and γ isoforms contain short inserts in their N-terminal regions (NTRs) that are not present in RCC1α. This region mediates interaction with chromatin, binds importin α3 and/ or importin β, and contains regulatory phosphorylation sites. RCC1γ is predominantly localised to the nucleus and mitotic chromosomes like RCC1α. However, compared to RCC1α, RCC1γ has a greatly reduced interaction with an importin α3-β and a stronger interaction with chromatin that is mediated by the extended NTR. RCC1γ is also the isoform that is most highly phosphorylated at serine 11 in mitosis. Unlike RCC1α, RCC1γ supports cell proliferation in tsBN2 cells more efficiently when serine 11 is mutated to non-phosphorylatable alanine. Phosphorylation. of RCC1γ therefore specifically controls its function during mitosis. These results show that human RCC1 isoforms have distinct chromatin binding properties, different molecular interactions, and are selectively regulated by pbosphorylation, as determined by their different NTRs.