Analysis of the effect of extracted yellow kepok banana peels (Musa paradisiaca l.) on the size and morphology of Enterococcus faecalis

Abstract

The treatment of pulp and periapical diseases often experience failure due to persistent infections in the pulp and periapical teeth. The dominant bacteria in pulp and periapical infections is Enterococcus faecalis. The use of antimicrobials has shifted to natural materials that have low toxicity, cheaper and have long-lasting properties. Yellow kepok banana peels have many antibacterial compounds such as flavonoids, tannins, alkaloids and easily found in Indonesia. The objective of this research was to determine the description of changes in the morphology and size of Enterococcus faecalis after giving the extract of Yellow kepok banana peels (Musa paradisiaca L) in vitro. The samples of the Yellow kepok banana peels extract with concentrations of 100%, 90%, 80%, 70%, 60% and control treatment were placed on wells with a coverslip. Each well was given BHI-B and Enterococcus faecalis. Coverslips at the 5 concentrations were coated using gold and photographed using a Scanning Electron Microscope (SEM). The photo results were then observed for the bacteria length and morphology. Data were analyzed with descriptive statistics. The average length of the bacteria given the extract increased, compared to the bacteria not given the extract. Meanwhile, the average width of the bacteria given the extract showed an uncertain change. The treated bacteria showed morphological changes in the form of blebs. In conclusion, extract of yellow kepok banana peels (Musa paradisiaca L.) provides an overview of the occurrence of changes in morphology and size of Enterococcus faecalis in vitro.