Cytokine-activated macrophages (MΦ) employ reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) to combat pathogens. The requirement for ROI for an effective host response to experimental leprosy using mice which have a disruption in the 91-kD subunit of the NAPDH oxidase cytochrome b (phox91-/-) was examined. Mycobacterium leprae multiplication in phox91-/- foot pads (FP) was elevated early in infection but subsequently arrested similarly to control mice within a noninvasive granuloma. Using a modified lepromin test model, a similar cellular composition in the M. leprae-induced FP granuloma in both strains with lymphocyte infiltration consisting primarily of CD4+CD44 hiCD62Llo effector cells was found. Of great interest was the disparity in the T cell population between the granuloma and the draining lymph node which contained predominantly naïve CD4+CD44 loCD62Lhi cells and was, therefore, not representative of the infection site. TH1 cytokines, chemokines and inducible nitric oxide synthase were comparably expressed in the FP of both strains. When infected in vitro, normal MΦ from B6 and phox91-/- mice supported bacterial viability, whereas IFNγ-activated MΦ killed M. leprae in a RNI-dependent manner, emphasizing that ROI was dispensable. These data show that phox91-/- mice generate a strong adaptive immune response and control long-term infection with M. leprae. © 2007 Federation of European Microbiological Societies.
CITATION STYLE
Hagge, D. A., Marks, V. T., Ray, N. A., Dietrich, M. A., Kearney, M. T., Scollard, D. M., … Adams, L. B. (2007). Emergence of an effective adaptive cell mediated immune response to Mycobacterium leprae is not impaired in reactive oxygen intermediate-deficient mice. FEMS Immunology and Medical Microbiology, 51(1), 92–101. https://doi.org/10.1111/j.1574-695X.2007.00282.x
Mendeley helps you to discover research relevant for your work.