Effect of defective interfering particles on plus- and minus- strand leader RNAs in vesicular stomatitis virus-infected cells

  • Leppert M
  • Kolakofsky D
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Abstract

Vesicular stomatitis virus-infected cells contain short RNA transcripts, called leader RNAs, which are coded by the exact 3' end of both the minus-strand and plus-strand nucleocapsid templates. The molar amounts of both the plus-strand leader RNA (which is templated from the minus-strand genome) and the minus-strand leader RNA (which is templated from the plus-strand antigenome) were determined both in standard-virus- and mixed-virus-infected cells by using end-labeled genome probes. The results demonstrate that the presence of defective interfering particles in the infecting virus stock decreases the amount of plus-strand leader RNA but increases the amount of minus-strand leader RNA found in the infected cells. In addition, considerably more minus-strand leader RNA per mole of nucleocapsid template is synthesized in mixed-virus-infected cells than plus-strand leader RNA per mole of nucleocapsid template in both standard-virus- and mixed-virus-infected cells.

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APA

Leppert, M., & Kolakofsky, D. (1980). Effect of defective interfering particles on plus- and minus- strand leader RNAs in vesicular stomatitis virus-infected cells. Journal of Virology, 35(3), 704–709. https://doi.org/10.1128/jvi.35.3.704-709.1980

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