Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously. Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before.
CITATION STYLE
Pfenninger, A., Karas, M., Finke, B., Stahl, B., & Sawatzki, G. (2001). Mass spectrometric investigations of human milk oligosaccharides. Advances in Experimental Medicine and Biology, 501, 279–284. https://doi.org/10.1007/978-1-4615-1371-1_35
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