The chapter deals with some biophysical methods used for investigating CPP-induced changes in membrane properties by spectroscopy methods such as fluorescence or NMR and methods used for probing CPP-induced leakage in membranes. Some useful model systems for biomembranes are described. These include large unilamellar phospholipid vesicles (LUVs) of well-defined size (diameter typically 100 nm). A protocol for the preparation of such vesicles is included. The leakage studies make use of LUVs with entrapped dye molecules. The NMR studies make use of mixed micelles (bicelles) as a membrane mimetic system, which can be oriented in the magnetic field of the spectrometer.
CITATION STYLE
Gräslund, A., & Mäler, L. (2011). Testing membrane interactions of CPPs. Methods in Molecular Biology (Clifton, N.J.), 683, 33–40. https://doi.org/10.1007/978-1-60761-919-2_3
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