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Isolation of Legionella pneumophila from nonepidemic-related aquatic habitats

Applied and Environmental Microbiology (1979) 37(6) 1239-1242
DOI: 10.1128/aem.37.6.1239-1242.1979
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Abstract

Continuous centrifugation of large volumes of water from natural southeastern lakes allowed quantitative detection of Legionella pneumophila by direct immunofluorescent staining. Positive samples were injected intraperitoneally into guinea pigs, and the L. pneumophila were isolated and identified by their morphological, cultural, physiological, and serological characteristics.

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Fliermans, C. B., Cherry, W. B., Orrison, L. H., & Thacker, L. (1979). Isolation of Legionella pneumophila from nonepidemic-related aquatic habitats. Applied and Environmental Microbiology, 37(6), 1239–1242. https://doi.org/10.1128/aem.37.6.1239-1242.1979

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