Monitoring and Visualization of Baculovirus Infection Using Green Fluorescent Protein Strategy

Abstract

Through green fluorescent protein (GFP) strategy, facile and fast monitoring and visualization of baculovirus infection in insect cells is possible in vivo. This chapter describes two novel techniques for simple determination of virus titer in the baculovirus expression system using GFP coexpression and rapid monitoring of infection of Sf-9 insect cells using combination of GFP and early-to-late (ETL) promoter. It is anticipated that the use of GFP under the control of ETL promoter will facilitate vector construction, virus isolation, and titer determination.