In the field of proteomics, numerous advanced technologies have evolved that aim to provide the molecular data necessary for an in-depth understanding of biological processes. Protein-protein interactions (PPI) are at the heart of cellular function and a milestone yet to be achieved is the mapping of a complete human interactome. Bioluminescence resonance energy transfer (BRET) has become a popular technique to investigate PPI. As BRET enables the detection of PPI in living cells, problems associated with in vitro biochemical assays can be circumvented, thus making BRET a powerful tool for monitoring interactions of virtually all kinds of protein species.
CITATION STYLE
Gersting, S. W., Lotz-Havla, A. S., & Muntau, A. C. (2012). Bioluminescence resonance energy transfer: An emerging tool for the detection of protein-protein interaction in living cells. Methods in Molecular Biology, 815, 253–263. https://doi.org/10.1007/978-1-61779-424-7_19
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