Effects of atorvastatin on autophagy in skeletal muscles of diabetic rats

Abstract

Aims/Introduction: Atorvastatin is usually used to decrease the amount of fatty substances in individuals with type 2 diabetes mellitus. However, it can cause side-effects, such as breakdown of skeletal muscle tissue. The present study focused on the effects of atorvastatin on autophagy of the skeletal muscles in diabetic rats. Materials and Methods: Diabetes in rats in the diabetic (D) and atorvastatin (T) groups was induced using streptozotocin (65 mg/kg, intraperitoneal injection). Next, rats in the T group were treated with atorvastatin (10 mg/kg/day, intragastric administration), whereas rats in the control and D groups were given water. Additionally, the rats in T and D groups were fed a high-fat and high-sugar diet for 10 weeks. Subsequently, the histopathological changes, and expression levels of microtubule-associated protein 1 light chain 3 (LC3)-I/-II and p62 in the skeletal muscle specimens in the three groups were analyzed. Results: Rats in the T group had reduced lipid droplets, cholesterol and low-density lipoprotein (P < 0.05) levels than those in the D group. Disordered atrophic myocytes, incrassated vascular walls and decreased cross-sectional area of type I fibers were found using hematoxylin–eosin and adenosine triphosphatase staining in the D and T groups. The messenger ribonucleic acid and protein levels of LC3-II and the LC3-II/LC3-I ratio were increased in the T group compared with those in the other groups (P < 0.05), whereas the protein level of p62 showed the opposite trend. Conclusions: Atorvastatin enhanced the autophagy level of skeletal muscles to decrease lipid deposition, which possibly exacerbated myopathy.