Among numerous heparin-binding proteins identified in animal tissues and body fluids, annexins are unique because their activies depend on their Ca2+ binding. Annexins are known to have other Ca2+-dependent activities. For example, they bind to phosphatidylserine in the plasma membrane, and some of them exhibit potent anticoagulant activity. This chapter describes three protocols that measure the Ca2+-dependent activities using recombinant annexins: solid-phase heparin-binding assay using bovine serum albumin-conjugated heparin, solid-phase phosphatidylserine-binding assay, and plasma coagulation inhibition assay.
CITATION STYLE
Nakayama, M., Tsunooka-Ohta, M., & Kojima-Aikawa, K. (2020). Annexin Lectins: Ca2+-Dependent heparin-binding activity, phosphatidylserine-binding activity, and anticoagulant activity. In Methods in Molecular Biology (Vol. 2132, pp. 661–668). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0430-4_57
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