Cytokinin Influence on Micropropagation System of Dianthus caryophyllus L

Abstract

A potent, quick and refined plant regeneration protocol was effectively established for Dianthus caryophyllus via nodal segment explants cut out from a 2-year-old vigorous mother plant. The three diverse cytokinins viz. 6-benzyladenine (BA), Kinetin (Kin) and thidiazuron (TDZ) were assessed as enhancements to Murashige and Skoog (MS) medium, the maximum frequency of multiple shoot bud induction was attained on MS medium augmented with 5.0 μM BA and 1.0 μM α-naphthalene-acetic acid (NAA). The in vitro raised shoots when subcultured on plant growth regulator (PGR) free MS basal medium extensively amplified the rate of shoot multiplication (44.8±3.5) and shoot length (7.5±0.29 cm) without showing any morphological and physiological features of degeneration even after fifth subculture passage. The in vitro raised microshoots were best rooted in half-strength MS medium augmented with 2.0 μM indole-3-butyric acid (IBA). The tissue culture raised plantlets with a completely developed shoot and root system were effectively transferred to the earthen pots having garden soil and grown in greenhouse condition with a 90% survival rate. Growth parameters of 2 months old in vitro raised plants were compared with in vivo grown seedlings of the same age group.