RAPD-PCR as a rapid approach for the evaluation of genotoxin-induced damage to bacterial DNA

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Abstract

RAPD PCR is a sensitive and reliable approach useful for the detection of DNA lesions due to environmental contaminants. In addition, this method is cost-effective, and can be performed in any laboratory having a DNA thermocycler and gel electrophoresis system. Here, we describe its application to identify genotoxin-induced DNA damage in foodborne bacteria. DNA alterations are detected through the analysis of electrophoresis profiles with the appearance or disappearance of new bands as compared to the non-mutated control. The described RAPD PCR procedure takes 6 h for completion. It uses small amounts of DNA and can reveal even low mutation rates.

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Tofalo, R., & Corsetti, A. (2017). RAPD-PCR as a rapid approach for the evaluation of genotoxin-induced damage to bacterial DNA. In Methods in Molecular Biology (Vol. 1644, pp. 195–201). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7187-9_18

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