Detection of protein glycation inhibitory potential of nine antidiabetic plants using a novel method

Abstract

Background: Protein glycation is a major cause of chronic diabetic complications. Medicinal plants with protein glycation inhibitory potential will be benefi cial to prevent or delay diabetic complications. Objective: The objective of the study was to analyze protein glycation inhibitory potential of ten plants among which nine are well known for their antidiabetic effects.Methods: Methanol extracts were prepared using parts from nine plants namely, Coccinia grandis, Ficus racemosa, Gymnema lactiferum, Gymnema sylvestre, Musa paradisiaca, Phyllanthus debilis, Phyllanthus emblica, Strychnos potatorum and Tinospora cordifolia. Dried latex of Pterocarpus marsupium was used without further extraction. Glycation inhibitory potential of the extracts was analyzed using bovine serum albumin (BSA) and fructose, incubated in the presence or absence of plant extracts for 4 week. Appropriate controls and blanks and the standard inhibitor aminoguanidine were included. A novel native polyacrylamide gel electrophoresis (PAGE) method established recently was used to detect the effect of plant extracts on the BSA glycation. Results: Previously we have demonstrated that the BSA migration towards the anode is increased proportionate to the degree of glycation. This increase was comparatively less when the glycation was inhibited. Accordingly, most promising antiglycation effects were observed with P. debilis (whole plant), P. emblica (fruit) and P. marsupium (latex). F. racemosa (stem bark) showed the highest inhibition among the remaining extracts. G. sylvestre (leaf), M. paradisiaca (yam) and T. cordifolia (leaf) also showed promising inhibition. C. grandis (leaf) and S. potatorum (seed) showed lower inhibition. Lowest inhibition was observed with G. lactiferum (leaf).Conclusion: We have demonstrated the antiglycation potential of some antidiabetic plants, using a novel method developed by us recently. Extracts of P. debilis, P. emblica and P. marsupium showed a strong protein glycation inhibitory potential.DOI: http://dx.doi.org/10.3126/ajms.v6i2.10584Asian Journal of Medical Sciences Vol.6(2) 2015 1-7