Cytoprotective Activity of Adhatoda Vasica Extract and Vasicine Against Tobacco Smoke Induced Cytoxicity

Abstract

The present study was undertaken to investigate the protection against cytotoxicity due to tobacco smoke by Adhatoda vasica and Vasicine. The antioxidant potential of AVE was analyzed through in vitro assays. The protective effect of Adhatoda vasica extract (AVE) and vasicine were analyzed in TSE treated group through MTT assay and microscopic analysis.A dose dependent increase in reducing power of AVE was observed. Treatment of A549 & THP-1 cell lines with 1-2 µg/ml (AVE) & 0.01-0.02 µg/ml (Vasicine) respectively for 3 hrs maintained the cell viability. Approximately 50% cell death was observed at 2% & 5% TSE on 24 hrs exposure. Pre-treatment of cell lines with AVE & Vasicine (2µg/ml & 0.02 µg/ml) respectively could overcome the toxic effect of TSE. This study showed that AVE has a great potential in reducing the toxic effects of A549 &THP-1 cell lines.