Effect of three different storage media on survival of periodontal ligament cells using collagenase-dispase assay

Abstract

Aim: To evaluate and compare the efficacy of propolis, egg albumen and Hank's balanced salt solution (HBSS) in maintaining the viability of human periodontal ligament (PDL) cells using a collagenase-dispase assay. Methodology: Fifty-five freshly extracted human teeth were divided into three experimental (HBSS, egg albumen and propolis) and two control groups. Fifteen teeth per experimental group were stored dry for 30 min and then immersed for 45 min in one of the three experimental media. The positive and negative controls corresponded to 0-min and 1-h dry time, respectively, with five teeth per control group. The teeth were then treated with collagenase II and dispase II for 30 min and labelled with 0.4% trypan blue for determination of viability. The number of viable cells was counted with a haemocytometer and analysed statistically by anova and post hoc Tukey HSD test. Results: Statistical analysis demonstrated there was no significant difference between HBSS, egg albumen and propolis in maintaining cell viability. Conclusion: Egg albumen and propolis may be able to maintain PDL cell viability as well as HBSS. © 2012 International Endodontic Journal.