Simultaneous Production of Muco- and Sulfated Polysaccharides by Marine Pseudomonas.

Abstract

A marine Pseudomonas species designated WAK-1 strain which was originally isolated from the brown seaweed Undaria pinnatifida produces an acidic muco (A-1)- and a sulfated (B-1) polysaccharides when grown in a seawater medium containing sucrose. Isolation and purification of these polysaccharides have been accomplished by precipitation with ethanol and Cetavlon, followed by DEAE-cellulose column chromatography. These polysaccharide preparations were homogeneous in both ultracentrifugal and electrophoretic analyses. A-1 was composed of galatosamine, glucuronic acid, and pyruvate at a molar ratio of 3 : 1 : 1, while B-1 was composed of galactose, glucose, and sulfate at a molar ratios of 2 : 1 : 2.