Estimation of total tannin and total phenolic content in plant (Crataegus azarolus L) by orbital shaker technique

Abstract

The plant of Crataegus Azarolus L, genus that belongs to the Rosaceae family and is a low, dense, spiny tree with a beautiful inflorescence, the phytochemical investigations on genus Crataegus were mainly performed on the leaves, flowers and berries. The objective of this experiment was to determine the ratio of tannin and phenol compound by method orbital shaker extraction. The plant of Crataegus azarolus L was collected in hasanbag mountain soran, Kurdistan. The parts of seed, leaf and steam of Crataegus Azarolus L was extract with water solvent and orbital shaker and assessed yield extraction, total tannin and total phenol. The highest significant value (p<0.05) of yield extraction was observed from leaf (71.56%), compared with other part of Crataegus Azarolus L. On the other hand, the total tannin was showed uppermost in leaf (3.03 kg/mg), and the steam (2.87 kg/mg). Whereas, the smallest total tannin was observed from the seed (1.10%). Furthermore, the total phenolic contents of the plant were observed the lowest value in seed as (0.98 mg GAE/g). Whereas, the total phenol in leaf was observed a higher significantly value (3.68 mg GAE/g) and in steam (1.92 mg GAE/g), with all significant value (p<0.05). This study showed that the leaf and other part of Crataegus Azarolus L, rich in total phenolic and total tannin after orbital shaker method was used.