In vitro-enhanced production of podophyllotoxin in phytohormonal-induced and regenerated roots of Podophyllum hexandrum

Abstract

Sixty-day-old in vitro-induced roots and roots regenerated from totipotent calli of Podophyllum hexandrum Royle produced an enhanced quantity of podophyllotoxin. Induced root cultures originated from aseptically germinated seedlings, and regenerated roots were established on both Gamborg's B5 and Murashige and Skoog media. The podophyllotoxin content was compared with normal plant root/rhizomes by HPLC. The highest values, both in growth/proliferation and in podophyllotoxin production, were obtained using Gamborg's B5 medium. © 2005 Taylor & Francis Ltd.