Localization of the phosphocholine-binding sites on C-reactive protein by immunoelectron microscopy.

  • Roux K
  • Kilpatrick J
  • Volanakis J
  • et al.
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Abstract

C-reactive protein (CRP) was reacted with monoclonal IgG antibody or Fab antibody fragments directed against the phosphocholine- (PC) binding site or a second unrelated site. The resulting immune complexes were viewed by a negative stain immunoelectron microscopy technique. Monoclonal anti-PC-binding site antibody bound to a single epitope on each of the five CRP subunits. The orientation of the PC-binding sites was determined to be slightly medial to one of the planar faces (A-face) of the molecule. The second monoclonal antibody, which was not PC-binding site related, bound to epitopes (one per CRP subunit) that were located slightly lateral to the other planar face (B-face) of the CRP molecule, i.e., opposite of the PC-binding site. Thus, the PC-binding site and the non-PC-binding site are oriented nearly perpendicular but on opposite sides with respect to the plane of the CRP molecule. The functional significance of this configuration is discussed.

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APA

Roux, K. H., Kilpatrick, J. M., Volanakis, J. E., & Kearney, J. F. (1983). Localization of the phosphocholine-binding sites on C-reactive protein by immunoelectron microscopy. The Journal of Immunology, 131(5), 2411–2415. https://doi.org/10.4049/jimmunol.131.5.2411

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