Effects of 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid (DIDS) and chlorpromazine on NO3- transport via anion exchanger in erythrocytes: Inertness of DIDS in whole blood

Abstract

We examined the effects of chlorpromazine on NO3- transport between erythrocytes (RBCs) and extracellular fluid. Chlorpromazine (10 μg/ml) did not influence NO3- movement in both whole blood and RBC suspension. Though an anion exchanger (AE1) inhibitor BIDS (4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid, 100 μM) did not alter NO3- movement in whole blood, it inhibited the movement in a concentration-dependent manner in the RBC suspension. The inhibition was abrogated by plasma and albumin concentration-dependently. Our results indicated that chlorpromazine had no effect on NO3- transport through AE1 and that the inertness of DIDS on AE1 in whole blood is due to interference by albumin in plasma.