There is a need for a general reporter system to study the interactions between a ligand and membrane protein in living cells. Here, we demonstrate a method that allows identification of undiscovered ligand partners of a membrane protein in its natural milieu. This assay will be of importance especially for multiple pass transmembrane proteins such as ion channels and GPCRs.
CITATION STYLE
Zhang, H., & Lerner, R. A. (2017). A Proximity-based assay for identification of ligand and membrane protein interaction in living cells. In Methods in Molecular Biology (Vol. 1575, pp. 215–222). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6857-2_13
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