A cationic tetraphenylethene as a light-up supramolecular probe for DNA G-quadruplexes

20Citations
Citations of this article
18Readers
Mendeley users who have this article in their library.

Abstract

Guanine-quadruplexes (G4s) are targets for anticancer therapeutics. In this context, human telomeric DNA (HT-DNA) that can fold into G4s sequences are of particular interest, and their stabilization with small molecules through a visualizable process has become a challenge. As a new type of ligand for HT-G4, we designed a tetraimidazolium tetraphenylethene (TPE-Im) as a water-soluble light-up G4 probe. We study its G4-binding properties with HT-DNA by UV-Visible absorption, circular dichroism and fluorescence spectroscopies, which provide insights into the interactions between TPE-Im and G4-DNA. Remarkably, TPE-Im shows a strong fluorescence enhancement and large shifts upon binding to G4, which is valuable for detecting G4s. The association constants for the TPE-Im/G4 complex were evaluated in different solution conditions via isothermal titration calorimetry (ITC), and its binding modes were explored by molecular modeling showing a groove-binding mechanism. The stabilization of G4 by TPE-Im has been assessed by Fluorescence Resonance Energy Transfer (FRET) melting assays, which show a strong stabilization (ΔT1/2 around +20◦ C), together with a specificity toward G4 with respect to double-stranded DNA.

Cite

CITATION STYLE

APA

Kotras, C., Fossépré, M., Roger, M., Gervais, V., Richeter, S., Gerbier, P., … Clément, S. (2019). A cationic tetraphenylethene as a light-up supramolecular probe for DNA G-quadruplexes. Frontiers in Chemistry, 7. https://doi.org/10.3389/fchem.2019.00493

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free