Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in pseudomonas syringae

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Abstract

Wedescribe the genetic organization of a copper-resistant plasmid containing copG and cusCBA genes in the plant pathogen Pseudomonas syringae. Chromosomal variants of czcCBA and a plasmid variant of cusCBA were present in different P. syringae pathovar strains. Transformation of the copper-sensitive Pseudomonas syringae pv. syringae FF5 strain with copG or cusCBA conferred copper resistance, and quantitative real-time PCR (qRT-PCR) experiments confirmed their induction by copper. © 2013, American Society for Microbiology.

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Gutiérrez-Barranquero, J. A., de Vicente, A., Carrión, V. J., Sundin, G. W., & Cazorla, F. M. (2013). Recruitment and rearrangement of three different genetic determinants into a conjugative plasmid increase copper resistance in pseudomonas syringae. Applied and Environmental Microbiology, 79(3), 1028–1033. https://doi.org/10.1128/AEM.02644-12

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