Identification of respiratory pathogen Bordetella Pertussis using integrated microfluidic chip technology

Abstract

Integrated PCR-CE chip technology has immense potential to be applied in clinical diagnostics. In this work we demonstrate the application of our integrated PCR-CE chip for the detection of the respiratory pathogen Bordetella pertussis. A series of experiments with varying cell concentrations (200,000-2 cfu) were performed to obtain the analytical detection limits of the chip. We find that the chip technology is well suited for sensitive detection of Bordetella pertussis, using genetic material from less than even 2 cfu. We also utilized an off-chip real-time PCR method to compare and validate our on-chip approach. © Springer-Verlag 2007.